{"id":3377,"date":"2017-03-29T10:58:17","date_gmt":"2017-03-29T08:58:17","guid":{"rendered":"https:\/\/www.toulouse-white-biotechnology.com\/insights\/actualites\/actualite\/nmr-and-molecular-interactions\/"},"modified":"2026-03-16T13:49:51","modified_gmt":"2026-03-16T12:49:51","slug":"nmr-and-molecular-interactions","status":"publish","type":"post","link":"https:\/\/www.toulouse-white-biotechnology.com\/en\/insights\/blog\/news\/nmr-and-molecular-interactions\/","title":{"rendered":"NMR and molecular interactions"},"content":{"rendered":"

\"\"Its mission<\/h2>\n\n

Understanding the functional dynamics of macromolecules as well as the modulation of their activity through interaction with metabolites.<\/p>\n\n

Key words<\/h2>\n\n

Nuclear Magnetic Resonance \u2013 molecular dynamics \u2013 molecular interactions<\/p>\n\n

Person in charge<\/h2>\n\nGuy Lippens<\/strong>\n\n

\u2013 Director of research CNRS<\/p>\n\nglippens@insa-toulouse.fr<\/a>\n\n

Team numbers in 2017: 1<\/h2>\n\n

Teachers and researchers: 1 \/ engineers\u00a0: 0 \/ assistant engineers and technicians\u00a0: 0 \/ post-doctoral and doctoral students: 0<\/p>\n\n

Thematic axes<\/h2>\n\n

NMR studies of enzymes, in particular their dynamic aspects and their regulation through interaction with metabolites<\/p>\n\n

Main activities<\/h2>\n\n
    \r\n \t
  • Methodologial developments in NMR<\/li>\r\n \t
  • Metabolites<\/li>\r\n \t
  • Structure and dynamics of macromolecules.<\/li>\r\n<\/ul>\n\n

    Research models<\/h2>\n\n
      \r\n \t
    • Purified molecules<\/li>\r\n \t
    • Bacteria<\/li>\r\n<\/ul>\n\n

      Fields of application and target products<\/h2>\n\n

      In collaboration with the research teams of the LISBP:<\/p>\n\n

        \r\n \t
      • Enzymes<\/li>\r\n \t
      • Metabolites<\/li>\r\n \t
      • Proteins that regulate glycolysis.<\/li>\r\n<\/ul>\n\n

        NMR, in addition to being a technique that provides structural information, enables a mapping of interactions, and this almost independently of affinity. Weak complexes (as enzyme\/substrate) therefore become accessible. The technique provides a dynamic image of the molecules.<\/p>\n\n

        Technologies, techniques, specific tools<\/h2>\n\n
          \r\n \t
        • Liquid NMR (in solution)<\/li>\r\n \t
        • NMR of cell suspensions.<\/li>\r\n<\/ul>\n\n

          Material<\/h3>\n\n
            \r\n \t
          • At Toulouse (LISBP)<\/strong>: spectrometer 800MHz equipped with a cryoprobe 1H\/13C\/15N\/31P (the possibility to observe the 31P being rather unique in France)<\/li>\r\n \t
          • At Lille<\/strong>: spectrometer 900MHz<\/li>\r\n \t
          • At Lyon<\/strong>: spectrometer 1GigaHz<\/li>\r\n<\/ul>\n\n

            Labelling<\/h3>\n\n
              \r\n \t
            • routine labelling with 15N and\/or 13C for proteins<\/li>\r\n \t
            • deuterium labelling (selective deuteration of amino acids) for larger systems (>100kDa).<\/li>\r\n<\/ul>\n\n

              \"\"\r\nIllustration: xylanase spectrum<\/em><\/p>\n\n

              Significant publications and patents<\/h2>\n\n

              Structure, Mechanism, and Antagonism of the Receptor Complex Mediated by Human TSLP in Allergy and Asthma. Verstraete K, Peelman F, Braun H, Lopez J, Van Rompaey D, Dansercoer A, Vandenberghe I, Pauwels K, Tavernier J, Devreese B, De Winter H, Beyaert R, Lippens G, Savvides SN. Nat Comm 2017, in press.\r\n\r\n15N-NMR-Based Approach for Amino Acids-Based 13C-Metabolic Flux Analysis of Metabolism. Millard P, Cahoreau E, Heuillet M, Portais JC, Lippens G. Anal Chem. 2017, 89(3):2101-2106.\r\n\r\nStudying Intrinsically Disordered Proteins under True In\u2005Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy. Lopez J, Schneider R, Cantrelle FX, Huvent I, Lippens G Angew Chem Int Ed Engl. 2016; 55(26):7418-22.\r\n\r\nCatalytic site inhibition of insulin-degrading enzyme by a small molecule induces glucose intolerance in mice. Deprez-Poulain R, Hennuyer N, Bosc D, Liang WG, En\u00e9e E, Marechal X,\r\nCharton J, Totobenazara J, Berte G, Jahklal J, Verdelet T, Dumont J, Dassonneville S, Woitrain E, Gauriot M, Paquet C, Duplan I, Hermant P, Cantrelle FX, Sevin E, Culot M, Landry V, Herledan A, Piveteau C, Lippens G, Leroux F, Tang WJ, van Endert P, Staels B, Deprez B. Nat Commun. 2015; 6: 8250.\r\n\r\nA Phosphorylation-Induced Turn Defines the Alzheimer's Disease AT8 Antibody Epitope on the Tau Protein. Gandhi NS, Landrieu I, Byrne C, Kukic P, Amniai L, Cantrelle FX, Wieruszeski JM, Mancera RL, Jacquot Y, Lippens G. Angew Chem Int Ed Engl. 2015;54(23):6819-23.<\/p>","protected":false},"excerpt":{"rendered":"

              Its mission Understanding the functional dynamics of macromolecules as well as the modulation of their activity through interaction with metabolites. Key words Nuclear Magnetic Resonance \u2013 molecular dynamics \u2013 molecular interactions Person in charge Guy Lippens \u2013 Director of research CNRS glippens@insa-toulouse.fr Team numbers in 2017: 1 Teachers and researchers: 1 \/ engineers\u00a0: 0 \/ […]<\/p>\n","protected":false},"author":1,"featured_media":3389,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_schema_org_content_json":"","footnotes":""},"categories":[1],"tags":[],"class_list":["post-3377","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3377","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/comments?post=3377"}],"version-history":[{"count":1,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3377\/revisions"}],"predecessor-version":[{"id":3386,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3377\/revisions\/3386"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media\/3389"}],"wp:attachment":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media?parent=3377"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/categories?post=3377"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/tags?post=3377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}