{"id":3505,"date":"2016-12-07T15:25:02","date_gmt":"2016-12-07T14:25:02","guid":{"rendered":"https:\/\/www.toulouse-white-biotechnology.com\/insights\/actualites\/actualite\/flow-cytometer-analyzer\/"},"modified":"2026-03-16T13:52:41","modified_gmt":"2026-03-16T12:52:41","slug":"flow-cytometer-analyzer","status":"publish","type":"post","link":"https:\/\/www.toulouse-white-biotechnology.com\/en\/insights\/blog\/news\/flow-cytometer-analyzer\/","title":{"rendered":"Flow cytometer analyzer"},"content":{"rendered":"<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-full wp-image-5990\" src=\"https:\/\/www.toulouse-white-biotechnology.com\/app\/uploads\/2016\/12\/cytometre-3.jpg\" alt=\"cytometre\" width=\"339\" height=\"339\"><\/figure>\n\n<strong>The cytometer MACSQuant\u00ae VYB (Miltenyi Biotec) enables an individual characterization of the fluorescence optical properties of cells in suspension.<\/strong>\n\n<p>This apparatus has recently been acquired by TWB to complete the\u00a0\u201cflow cytometry\u201d\u00a0service offer. It is located on the Biotransformation &amp; culture platform, under the responsibility of Delphine LESTRADE. The flow cytometer MACSQuant\u00ae VYB (Miltenyi Biotec) is a<\/p>\n\n<strong>cytometer-analyzer enabling an individual, quantitative and qualitative characterization of fluorescence optical properties of cells in suspension<\/strong>\n\n<p>: microbial cells or lower eukaryotic or microalgae cells for example. It is an automated instrument, equipped with a sample changer, which<\/p>\n\n<strong>can analyze up to 10 000 events per second<\/strong>\n\n<p>.\r\n\r\nThe flow cytometry technique enables the high-speed streaming of cells. Each particle can be excited by one or several lasers. Many detectors placed at different angles provide information in terms of size, structure, and fluorescent content for each cell.<\/p>\n\n<strong>The MACSQuant VYB is equipped with three monochromatic excitation lasers, ten optical detection channels<\/strong>\n\n<p>and an intuitive software to facilitate a quick and simple cellular analysis. This instrument is versatile for<\/p>\n\n<strong>phenotypic and\/ or functional analyses using fluorescent reporters<\/strong>\n\n<p>\u00a0such as dyes, fluorescent proteins or antibodies coupled to fluorochromes. It is configured with a 561nm laser for an improved and more sensitive analysis of the fluorescent proteins located in the yellow \/ green excitation range.\r\n\r\nThe \u201csample changer\u201d\u00a0platform is automated and flexible, thus enabling the sequence analysis of samples packed in 5 mL, 15 mL, or 50mL tubes but also in 96-well plate. The sample injection is performed through a 500 mL Hamilton syringe. Since the sample volume is precisely known, it is possible to obtain an<\/p>\n\n<strong>enumeration of the different cell populations<\/strong>\n\n<p>observed. The concentration will be given in number of cells per mL with a +\/- 2% accuracy.<\/p>\n\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-5989\" src=\"https:\/\/www.toulouse-white-biotechnology.com\/app\/uploads\/2016\/12\/cytometre-2-1.jpg\" alt=\"cytometre-2\" width=\"890\" height=\"593\">\r\n<em>The cytometer MACSQuant\u00ae VYB Miltenyi Biotec (Credits: TWB \/ B. Hamousin)<\/em><\/p>\n\n<h2 class=\"wp-block-heading\">The characteristics of the MACSQuant VYB cytometer are<\/h2>\n\n<ul class=\"wp-block-list\">\r\n \t<li>3 monochromatic lasers: 405, 488 and 561nm<\/li>\r\n \t<li>10 detection\u00a0channels: including 2 relating to cell size (FSC) and cellular complexity (SSC) + 8 dedicated to fluorescence detection (see optical diagram Fig 1 below)<\/li>\r\n \t<li>Minimum volume of analysis: 25\u00b5L<\/li>\r\n \t<li>resolution: 0.5 \u00b5m<\/li>\r\n<\/ul>\n\n<h2 class=\"wp-block-heading\">It is possible<\/h2>\n\n<ul class=\"wp-block-list\">\r\n \t<li>to use the sample changer for a fully automated analysis (tube changer 5 ml; 15ml; 50ml and 96-well plate changer)<\/li>\r\n \t<li>to perform a labeling with incubation management before the analysis and using up to 4 different reagents<\/li>\r\n \t<li>to maintain an analysis temperature at 4\u00b0C for 4h<\/li>\r\n \t<li>to analyze cells between 0.5 \u00b5m and 150 \u00b5m in size.<\/li>\r\n<\/ul>\n\n<h2 class=\"wp-block-heading\">Examples of typical applications<\/h2>\n\n<ul class=\"wp-block-list\">\r\n \t<li>Direct count of microorganisms<\/li>\r\n \t<li>Detection and count of viable, viable but non-cultivable, and dead microorganisms<\/li>\r\n \t<li>Quantification of reporter genes<\/li>\r\n \t<li>Characterization of physiological data: intracellular pH, oxidative stress, membrane fluidity, energy charge, membrane potential.<\/li>\r\n \t<li>Fine analysis of microbial catalysts for process improvement<\/li>\r\n \t<li>Cell bank screening<\/li>\r\n \t<li>Assessment of the populations in microbial consortia.<\/li>\r\n<\/ul>\n\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-5991\" src=\"https:\/\/www.toulouse-white-biotechnology.com\/app\/uploads\/2016\/12\/schema-cytometre-1024x653-2.png\" alt=\"schema-cytometre\" width=\"680\" height=\"434\">\r\n<em>Fig. 1: Diagram of the fluorescence detection optical bench of MACSQuant VYB (Miltenyi Biotec)<\/em><\/p>\n\n<em>Contact: Delphine Lestrade, Manager cytometer TWB (<a href=\"mailto:delphine.lestrade@inra.fr\">delphine.lestrade@inra.fr<\/a>)<\/em>","protected":false},"excerpt":{"rendered":"<p>The cytometer MACSQuant\u00ae VYB (Miltenyi Biotec) enables an individual characterization of the fluorescence optical properties of cells in suspension. This apparatus has recently been acquired by TWB to complete the\u00a0\u201cflow cytometry\u201d\u00a0service offer. It is located on the Biotransformation &amp; culture platform, under the responsibility of Delphine LESTRADE. The flow cytometer MACSQuant\u00ae VYB (Miltenyi Biotec) is [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":3603,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_schema_org_content_json":"","footnotes":""},"categories":[1],"tags":[],"class_list":["post-3505","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/comments?post=3505"}],"version-history":[{"count":1,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3505\/revisions"}],"predecessor-version":[{"id":3595,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3505\/revisions\/3595"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media\/3603"}],"wp:attachment":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media?parent=3505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/categories?post=3505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/tags?post=3505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}