{"id":3763,"date":"2016-03-30T22:06:15","date_gmt":"2016-03-30T20:06:15","guid":{"rendered":"https:\/\/www.toulouse-white-biotechnology.com\/insights\/actualites\/actualite\/metabolism-of-prokaryotes\/"},"modified":"2026-03-16T13:52:50","modified_gmt":"2026-03-16T12:52:50","slug":"metabolism-of-prokaryotes","status":"publish","type":"post","link":"https:\/\/www.toulouse-white-biotechnology.com\/en\/insights\/blog\/news\/metabolism-of-prokaryotes\/","title":{"rendered":"Metabolism of prokaryotes\u00a0"},"content":{"rendered":"http:\/\/www.lisbp.fr\/en\/research\/metabolism-of-prokaryotes–ead4.html<\/a>\n\n

Its mission<\/h2>\n\n

Understanding the metabolic regulations involved in the adaptation of bacteria in industrial environments to improve their performances. Optimizing the expression of metabolic pathways. (Read more)<\/p>\n\n

Key words<\/h2>\n\n

Bacterial adaptation\u00a0\u2013 Systems and synthetic biology – Biodiversity<\/p>\n\n

Person in charge<\/h2>\n\nMuriel Cocaign-Bousquet<\/strong>\n\n

\u2013 Director of research INRA<\/p>\n\ncocaign@insa-toulouse.fr<\/a>\n\n

Team numbers in 2016: 16<\/h2>\n\n

Teachers and researchers: 7 \/ engineers\u00a0: 2 \/ assistant engineers and technicians\u00a0: 3 \/ post-doctoral and doctoral students: 2<\/p>\n\n

Thematic axes<\/h2>\n\n
    \r\n\t
  • Optimization of bacterial performances by nutritional or metabolic engineering strategies<\/li>\r\n\t
  • Optimization of gene expression: RNA\u00a0dynamics and their\u00a0control, a leverage point still under-explored<\/li>\r\n\t
  • Analysis of endogenous regulation networks and of the dialogue with artificial\u00a0metabolic pathways<\/li>\r\n\t
  • Genomic and functional biodiversity: a reservoir\u00a0for innovation<\/li>\r\n<\/ul>\n\n

    Main activities<\/h2>\n\n
      \r\n\t
    • \u00ab Omic \u00bb scale analysis of bacterial metabolism and its regulations (multi-scale approach)<\/li>\r\n\t
    • Genotypic and phenotypic screenings (natural biodiversity analysis)<\/li>\r\n\t
    • Dynamic analysis of bacterial performances under conditions\u00a0of stress and industrial constraints<\/li>\r\n\t
    • Optimization of bacterial performances and of new metabolic pathways\u2019 expression<\/li>\r\n<\/ul>\n\n

      Research models<\/h2>\n\n

      Several bacteria of industrial interest<\/p>\n\n

        \r\n\t
      • The model bacterium Escherichia coli<\/li>\r\n\t
      • Various lactic\u00a0acid bacteria and more particularly Lactococcus lactis<\/li>\r\n\t
      • Different pathogenic species (C. diphtheriae, H. influenzae, B. pertussis, etc.)<\/li>\r\n<\/ul>\n\n

        Fields of application and target products<\/h2>\n\n
          \r\n\t
        • Agribusiness (new foods, dairy and cheese products, probiotics, flavours)<\/li>\r\n\t
        • Pharmaceutical industry (production of vaccines)<\/li>\r\n\t
        • Industrial biotechnology (heterologous proteins)<\/li>\r\n<\/ul>\n\n

          \"CaptureTechnologies, techniques, specific tools<\/h2>\n\n
            \r\n\t
          • Molecular typing by MLST<\/li>\r\n\t
          • Measuring gene expression: transcriptome ([transcripts]), stabilome (mRNA stability), translatome (ribosome density)<\/li>\r\n\t
          • Analysis techniques and genome modifications<\/li>\r\n\t
          • Measuring enzyme and metabolite activities<\/li>\r\n\t
          • Bacterial culture (continuous and discontinuous)<\/li>\r\n\t
          • Phenotyping<\/li>\r\n\t
          • Development of mathematical tools for integrating\u00a0and modelling multi-scale data<\/li>\r\n<\/ul>\n\n

            Significant publications and patents<\/h2>\n\n
              \r\n\t
            • Esquerr\u00e9 T, Laguerre S, Turlan C, Carpousis AJ, Girbal L, Cocaign-Bousquet M. 2014. Dual role of transcription and transcript stability in the regulation of gene expression in Escherichia coli<\/em> cells cultured on glucose at different growth rates. Nucleic Acids Res. 42:2460-2472.<\/li>\r\n\t
            • Nouaille S, Rault L, Jeanson S, Loubi\u00e8re P, Le Loir Y, Even S. 2014. Contribution of Lactococcus lactis<\/em> reducing properties to the downregulation of a major virulence regulator in Staphylococcus aureus<\/em>, the agr system. Appl Environ Microbiol. 80(22):7028-35.<\/li>\r\n\t
            • LeHir J, P Loubi\u00e8re, F Barbirato, ND Lindley. Method for producing Haemophilus<\/em> influenzae type B<\/em> antigens. Brevet\u00a0 WO2014\/006318, Jan 9, 2014.<\/li>\r\n\t
            • Saulou-B\u00e9rion C, Gonzalez I, Enjalbert B, Audinot J-N, Fourquaux I, Jamme F, Cocaign-Bousquet M, Mercier-Bonin M, and Girbal L. 2015. Escherichia coli<\/em> facing ionic silver stress: an integrative approach to explore the transcriptional, physiological and biochemical responses.\u00a0PLoS ONE 10(12): e0145748<\/li>\r\n\t
            • Izac M, Garnier D, Speck D, Lindley ND. 2015. A Functional Tricarboxylic Acid Cycle Operates during Growth of Bordetella pertussis<\/em> on Amino Acid Mixtures as Sole Carbon Substrates. PLoS ONE 10(12): e0145251.<\/li>\r\n<\/ul>\n\n

              Precompetitive projects<\/h2>\n\nAPPOSA<\/strong>\n\n

              – Improvement of targeted protein production by optimized transcripts stability<\/p>\n\nINSEREE<\/strong>\n\n

              – Expression engineering of E. coli strain for enzymatic models with an unnatural amino acid<\/p>","protected":false},"excerpt":{"rendered":"

              http:\/\/www.lisbp.fr\/en\/research\/metabolism-of-prokaryotes–ead4.html Its mission Understanding the metabolic regulations involved in the adaptation of bacteria in industrial environments to improve their performances. Optimizing the expression of metabolic pathways. (Read more) Key words Bacterial adaptation\u00a0\u2013 Systems and synthetic biology – Biodiversity Person in charge Muriel Cocaign-Bousquet \u2013 Director of research INRA cocaign@insa-toulouse.fr Team numbers in 2016: 16 Teachers […]<\/p>\n","protected":false},"author":1,"featured_media":3815,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_schema_org_content_json":"","footnotes":""},"categories":[1],"tags":[],"class_list":["post-3763","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3763","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/comments?post=3763"}],"version-history":[{"count":1,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3763\/revisions"}],"predecessor-version":[{"id":3801,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/posts\/3763\/revisions\/3801"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media\/3815"}],"wp:attachment":[{"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/media?parent=3763"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/categories?post=3763"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.toulouse-white-biotechnology.com\/en\/wp-json\/wp\/v2\/tags?post=3763"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}